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	Provedor de dados Genet. Mol. Biol. (2) Naturalis (1) Acta Botanica (1) Biota Neotropica (1) Ciência Rural (1) Autor Arntzen, J.W. (1) Backes,Alice (1) Barros,Fábio de (1) Biondo,Cibele (1) Caparroz,Renato (1) Cozzolino,Salvatore (1) Favoreto,Fernanda Campanharo (1) Freitas,Loreta B. (1) Gontijo,Andreia Barcelos Passos Lima (1) Gutiérrez-Rodríguez, J. (1) Leite,Natalia Gonçalves (1) Lima,Ed Christian Suzuki de (1) Lopera-Barrero,Nelson Mauricio (1) Martínez-Solano, I. (1) Miranda,Fabio Demolinari de (1) Miyaki,Cristina Y. (1) Mäder,Geraldo (1) Oliveira-Marques,Adriana R. (1) Palma-Silva,Clarisse (1) Pandolfi,Victor César Freitas (1) Mais... Palavra-chave Cross-amplification (6) Conservation (3) Genetic diversity (2) SSR (2) Amphibians (1) Bromeliads (1) Epidendrum (1) Gene flow (1) Genetic polymorphism (1) Hybridization (1) Macaw (1) Microsatellite (1) Microsatellites (1) Molecular markers (1) Morphology (1) Orchidaceae (1) Plastid haplotypes (1) Population genetics (1) Psittaciformes (1) SSR markers (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Article / Letter to the editor (1) Info:eu-repo/semantics/other (1) Ano 2019 (1) 2018 (1) 2017 (1) 2012 (1) 2011 (1) 2009 (1) Mais... País Brazil (5) Netherlands (1) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Comparative analysis of microsatellite variability in five macaw species (Psittaciformes, Psittacidae): application for conservation Genet. Mol. Biol. Presti,Flavia T.; Oliveira-Marques,Adriana R.; Caparroz,Renato; Biondo,Cibele; Miyaki,Cristina Y.. Cross-amplification was tested and variability in microsatellite primers (designed for Neotropical parrots) compared, in five macaw species, viz., three endangered blue macaws (Cyanopsitta spixii [extinct in the wild], Anodorhynchus leari [endangered] and Anodorhynchus hyacinthinus [vulnerable]), and two unthreatened red macaws (Ara chloropterus and Ara macao). Among the primers tested, 84.6% successfully amplified products in C. spixii, 83.3% in A. leari, 76.4% in A. hyacinthinus, 78.6% in A. chloropterus and 71.4% in A. macao. The mean expected heterozygosity estimated for each species, and based on loci analyzed in all the five, ranged from 0.33 (A. hyacinthinus) to 0.85 (A. macao). As expected, the results revealed lower levels of genetic variability... Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Cross-amplification; Macaw; Microsatellite; Psittaciformes. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200030 Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum Genet. Mol. Biol. Pinheiro,Fábio; Palma-Silva,Clarisse; Barros,Fábio de; Cozzolino,Salvatore. In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus. Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidendrum; Orchidaceae; Short tandem repeat; Cross-amplification. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021 Genetic diversity in Calibrachoa pygmaea (Solanaceae): A hawkmoth-pollinated nightshade from the Pampas Acta Botanica Mäder,Geraldo; Backes,Alice; Reck-Kortmann,Maikel; Freitas,Loreta B.. ABSTRACT Calibrachoa pygmaea is a unique species of Calibrachoa, especially concerning its flower morphology and the environment where it occurs. The species is self-incompatible and is narrowly distributed in wet and flooded fields of the Pampas region. We characterize the genetic diversity of the species based on traditional plastid markers and newly developed nuclear microsatellites to identify drivers that guide its evolution. Our results identified markers that are informative and useful for studying the population structure of C. pygmaea, as well as that of other species of Calibrachoa. Both marker sets were congruent in developing conclusions regarding the evolutionary scenario of C. pygmaea, and revealed that the genetic variability and population... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cross-amplification; Gene flow; Genetic diversity; Plastid haplotypes; Population genetics; SSR markers; Wild petunias. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400664 Morphological and molecular data to describe a hybrid population of the Common toad (Bufo bufo) and the Spined toad (Bufo spinosus) in western France Naturalis Trujillo, T.; Gutiérrez-Rodríguez, J.; Arntzen, J.W.; Martínez-Solano, I.. The use of hyper-variable markers across species is often hindered by low cross-species amplification success, a reduced level of polymorphism or a high frequency of null alleles. However, optimizing sets of reliable and informative markers that can be consistently amplified and scored across taxa is key to address questions about patterns of genetic diversity and structure, hybridization and speciation. Here we present 14 newly developed microsatellite markers in the Spined toad (Bufo spinosus), assess their polymorphism in two Iberian populations and test for cross-species amplification in the closely related Common toad (Bufo bufo). We then use the 12 loci co-amplifying in both species to the study of a morphologically intermediate population (Moyaux)... Tipo: Article / Letter to the editor Palavras-chave: Amphibians; Cross-amplification; Genetic polymorphism; Hybridization; Microsatellites; Morphology. Ano: 2017 URL: http://www.repository.naturalis.nl/record/623750 Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae Biota Neotropica Miranda,Fabio Demolinari de; Gontijo,Andreia Barcelos Passos Lima; Santiliano,Fabiano Costa; Favoreto,Fernanda Campanharo; Soares,Taís Cristina Bastos. Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.)... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cross-amplification; SSR; Genetic diversity; Bromeliads; Conservation. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032 Transferability of heterologous microsatellite primers in Brycon gouldingi Ciência Rural Souza,Felipe Pinheiro de; Lima,Ed Christian Suzuki de; Leite,Natalia Gonçalves; Urrea-Rojas,Angela Maria; Yamachita,Andrei Lincoln; Pandolfi,Victor César Freitas; Lopera-Barrero,Nelson Mauricio. ABSTRACT: Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Cross-amplification; Molecular markers; SSR. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100406 Registros recuperados: 6 Primeira ... 1 ... Última

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